Glyco-regioisomerism Effect on Lectin-Binding and Cell-Uptake Pathway of Glycopolymer-Containing Nanoparticles【ALV·复旦】
Glyco-regioisomerism Effect on Lectin-Binding and Cell-Uptake Pathway of Glycopolymer-Containing Nanoparticles
作者P Sun，Y He，M Lin，Y Zhao，Y Ding，...
摘要Sugar regioisomerism (glycosidic linkage on different hydroxyl groups of the same sugar) widely exists in various polysaccharides and glycans with a significant contribution to their biological functions. However, the effects of this regioisomersim in glycopolymers and their self-assembled nanoparticles on such functions were almost not investigated previously. In this paper, this regioisomersim effect is studied for self-assembled nanoparticles NP-1-Gal and NP-6-Gal from triblock copolymers carrying different constitutional isomers of the pendent sugar species (1 and 6 denote the glycosidic linkage from the anomeric position and 6 position of the galactose unit, respectively). NP-1-Gal shows strong binding to lectins of Peanut (Arachis hypogea) agglutinin (PNA) and Erythrina cristagalli agglutinin (ECA), while NP-6-Gal does not. More importantly, they show binding behavior similar to the asialoglycoprotein receptor (ASGPR) but different internalization pathways in the Hep G2 cell after ASGPR-mediated end...收起