本文为中科院生态环境研究中心的研究学者发表在《Journal of Environmental Sciences》 2012年12期的文章，题目为Enantioseletive bioaccumulation of tebuconazole in earthworm Eisenia fetida。
【摘要】：Methods of extraction and determination of tebuconazole enantiomers in earthworm(Eisenia fetida)were developed by capillary electrophoresis(CE)and high performance liquid chromatography(HPLC).Both CE and HPLC have excellent resolution and recovery.The linearity ranges were 2.9-102.4mg/kg and 3.0-99.6mg/kg for(+)-R-tebuconazole and(-)-S-tebuconazole respectively in CE,and from 0.56 to 1000mg/kg for both enantiomers in HPLC.Enantioselective bioaccumulation in earthworms from soil was investigated under laboratory condition at concentrations of 10 and 50 mg/kg dw in soil.The uptake kinetics of(+)-R-tebuconazole fitted the firstorder kinetics well with r2 0.97 and 0.94 under 10 and 50mg/kg dw exposure condition,respectively,while(-)-S-tebuconazole with r 2 0.75 and 0.22 did not show the same.Bioaccumulation of tebuconazole in earthworm tissues was enantioselective with a preferential accumulation of(+)-R-tebuconazole.The(+)-R-tebuconazole might also have biomagnifying effect potential in earthworm food chain with biota-sediment accumulation factor(BSAF) of 1.64kg OC/kg lip in 10 mg/kg dw exposure group and 2.61kg OC/kg lip in 50mg/kg dw exposure group from soil to earthworm after 36 days.Although(-)-S-tebuconazole shares the same physicochemical properties with(+)-R-tebuconazole,it did not biomagnify.BSAFs of(-)-S-tebuconazole were 0.50kg OC/kg lip(10 mg/kg dw tebuconazole exposure) and 0.28kg OC/kg lip(50mg/kg dw tebuconazole exposure) after 36 days,which was possibly owing to biotransformation or metabolism in earthworm tissues.
【关键词】：tebuconazole;earthworm;bioaccumulation;enantioselectivity 【基金】：supported by the Innovative Program of the Chinese Academy of Sciences (No. KZCX2-YW-JS403) the National High Technology Research and Development Program (863) of China (No. 2010AA065105)